NR AXSJ
AU Mathiason,C.K.; Powers,J.G.; Dahmes,S.J.; Osborn,D.A.; Miller,K.V.; Warren,R.J.; Mason,G.L.; Hays,S.A.; Hayes-Klug,J.; Seelig,D.M.; Wild,M.A.; Wolfe,L.L.; Spraker,T.R.; Miller,M.W.; Sigurdson,C.J.; Telling,G.C.; Hoover,E.A.
TI Detection of Chronic Wasting Disease Prions in Saliva, Blood, and Excreta of Deer
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.106
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Background: The potential presence of prions in body fluids is perhaps most relevant to chronic wasting disease (CWD) of cervids, owing to its facile transmission, geographic expansion, and the relatively large amount of aberrant prion protein in peripheral lymphoid tissues. Nevertheless the exact mode by which the CWD prions are shed and transmitted has remained unknown.
Objective: To determine whether infectious CWD prions are present in saliva, blood or urine and feces of CWD-positive deer.
Methods: Two bioassay studies comprising three cohorts for a total of n = 6 naive deer/cohort were exposed either orally to 50 ml saliva, or 50 ml urine and 50 gram feces, or via intravenous transfusion of 250 ml whole blood from CWD-positive deer. Study controls included positive control cohorts totalling (n = 8) deer exposed to brain from CWD-positive deer and a negative control cohort consisting of (n = 2) deer receiving inocula from CWD-negative deer. The recipient animals were maintained under rigorous indoor isolation conditions to exclude potential adventitious prion exposure and monitored for CWD infection for a minimum of 18 months post infection by serial tonsil biopsy and terminal necropsy.
Results: Infectious prions capable of transmitting CWD were detected in saliva (by the oral route) and in blood (by transfusion). PrPcWD was first detected in tonsils between 3 and 12 months post inoculation. To our surprise, no deer fed urine and feces from CWD-positive donors developed CWD infection, despite multiple exposures.
Conclusion: Infectious prions in saliva may explain the efficient transmission of CWD in nature. Infectious prions in the blood of CWD-positive deer establishes a basis for developing antemortem detection of the disease by blood-based assay methods and emphasizes the widespread distribution of infectivity in CWD-positive deer.
AD C. Mathiason, G. Mason, S. Hays, J. Hayes-Klug, D. Seelig, C. Sigurdson, E. Hoover, Colorado State University, Microbiology, Immunology and Pathology, USA; J. Powers, M. Wild, National Park Service, Biological Resource Management Division, USA; S. Dahmes, Wildlife Artist Supply Company (WASCO, Inc.), USA; D. Osborn, K. Miller, R. Warren, University of Georgia, Athens, Warnell School of Forestry and Natural Resources, USA; L. Wolfe, M. Miller, Colorado Division of Wildlife, Wildlife Research Center, USA; T. Spraker, Colorado State University, Veterinary Diagnostic Laboratory, CVMBS, USA; G. Telling, University of Kentucky, Microbiology, Immunology and Molecular Genetics, USA
SP englisch
PO Schottland
EA pdf-Datei und Poster (Postertitel: Additional bioassay studies to detect CWD prions in saliva, blood and excreta of deer)