NR AXTH
AU Murdoch,B.; McKay,S.D.; Prasad,A.; Marques,E.; Kolbehdari,D.; Wang,Z.; Stella,A.; Clawson,M.L.; Heaton,M.P.; Laegreid,W.W.; Moore,S.S.; Williams,J.L.
TI Genome Scan for BSE Susceptibility and/or Resistance in European Holstein Cattle
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.126
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Genetic susceptibility to various transmissible spongiform encephalopathies (TSEs) has been observed in many species including mice, humans and sheep. Evidence for susceptibility genes on several bovine chromosomes has been reported. More recently there have been reports of associations between disease incidence and insertion/deletion variants within the promoter region of the prion gene (PRNP) itself. The objective of this study was to perform a genome scan to test for genetic associations with bovine spongiform encephalopathy (BSE) susceptibility and/or resistance in European Holstein cattle. Optimized assays with 3072 genome wide single nucleotide polymorphism (SNP) with and average of 100 markers per chromosome were genotyped across 812 BSE Holstein cattle, including BSE positive and control animals. In total approximately 2.5 million genotypes were determined. Significant SNP single marker associations were observed on three chromosomes previously reported to be associated with BSE resistance/susceptibility as well as several new chromosomes. Variations at 27 chromosomal regions were associated with disease, 31 SNPs with a p<0.01. Of these associations 20 SNPs on 11 chromosomes had a p<0.005. A total of 8 SNPs on 7 chromosomes (BTA 4, 10, 14, 15, 21, 25, 28) had a p<0.001 of which 3 SNPs (BTA 4, 14, 21) had a p<0.0005. It should be noted that additional marker and or tests are required to verify the biological significance of these associations. An additional 17 haplotype tagging SNPs (htSNPs) were genotyped within the PRNP locus. Preliminary analysis indicated an association between PRNP htSNP 4136 (located in the promoter region) and BSE resistance (dominance effect, P=0.025, additive effect, P=0.0078). However many alleles in this region have been reported to be in strong linkage disequilibrium. The preliminary results of this study indicate that variations, not only within the promoter region of the PRNP gene, but at other loci throughout the genome have an association with BSE disease in European Holstein cattle.
AD B. Murdoch, S.D. McKay, A. Prasad, E. Marques, D. Kolbehdari, Z. Wang, S.S. Moore, University of Alberta, Agricultural, Food, and Nutritional Science, Canada; A. Stella, J.L. Williams, Polo Universitario, Parco Tecnologico Padano, Italy; M.L. Clawson, M.P. Heaton, W.W. Laegreid, United States Department of Agriculture, Meat Animal Research Center, USA
SP englisch
PO Schottland