NR AXTN
AU Nentwig,A.; Oevermann,A.; Heim,D.; Botteron,C.; Zellweger,K.; Drögemüller,C.; Zurbriggen,A.; Seuberlich,T.
TI Molecular and Neuropathological Characterization of Small Ruminant TSE Cases in Switzerland
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Natural and Experimental Strains P02.01
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB In recent years cases of transmissible spongiform encephalopathies (TSE) were identified in small ruminants (SR) that differed from classical scrapie in the molecular characteristics of the disease associated proteinase K-resistant part of the pathological prion protein (PrPsc), PrPres. In Switzerland, nine cases of SR TSE were identified in the years 2004-2005 by active and passive surveillance. In this study we analyze the molecular and neuropathological phenotype of these cases in detail. One sheep was identified as classical scrapie, whereas six sheep as well as two goats were classified as atypical scrapie. The latter revealed an uniform PrPres electrophoretic mobility pattern distinct from classical scrapie and bovine spongiform encephalopathy regardless of the genotype, the species and the neuroanatomical structure. Remarkably different types of neuroanatomical PrPsc distribution were observed in atypical scrapie cases by both Western immunoblotting and immunohistochemistry. Our findings suggest that the phenotype variation in atypical scrapie is larger than expected and thus impact on disease surveillance strategies.
AD A. Nentwig, A. Oevermann, C. Botteron, K. Zellweger, A. Zurbriggen, T. Seuberlich, University of Berne, NeuroCenter, Switzerland; D. Heim, Swiss Federal Veterinary Office, Switzerland; C. Drögemüller, University of Berne, Institute of Genetics, Switzerland
SP englisch
PO Schottland