NR AXTW
AU Oelschlegel,A.M.; Geissen,M.; Riebe,R.; Schätzl,H.M.; Groschup,M.H.
TI Bov5 Cells: A Permanent Bovine Cell Line which is Susceptible to Ovine Scrapie
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.03
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Only few stably prion infected cell lines are available to date and these lines are almost exclusively coming from rodent species. Apart from these lines a large collection of the commonly used as well as many unknown permanent cell lines have been tested without finding a susceptible cell line, e.g. from ruminant species. The factors necessary for a proficient replication as well as for determining strain effects are still unknown. In the study presented here, we were able to infect a non-transgenic bovine cell line (Bov5) with natural sheep scrapie prions. Scrapie infected Bov5 cells (Bov5Sc) were consistently passaged. The accumulation of PrPres could be increased by selection and PrPres was still detectable after more than 200 passages. PrPres signals were detected by dot-blot, western-blot and by a "Cell ELISA". PrPres from Bov5Sc cells showed a similar PK resistance as PrPres derived from murine RML infected cell lines (ScN2a, SMBRC040). However, Bov5Sc were only extremely slowly cured by chemical compounds known to effectively inhibit the prion replication, e.g. Suramin took more than 4 weeks to cure the cells. However, cured cells could be reinfected with a second field scrapie isolate. The propagation of infectivity in Bov5Sc cells was verified by mouse bioassays (Tgbov XV mice, Tgshp XI mice).
AD A. Oelschlegel, M. Geissen, M.H. Groschup, Friedrich-Löffler-Institut, Germany; R. Riebe, Friedrich-Löffler-Institut, Germany; H.M. Schätzl, TUM, Germany
SP englisch
PO Schottland