NR AXTX
AU Oh,J.M.; Choi,E.K.; Carp,R.I.; Kim,Y.S.
TI The Cellular Prion Protein Prevent Autophagic Cell Death in Neuronal Cells
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.35
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB The cellular prion protein (PrPc) is abundantly expressed in neurons of the central nervous system. One of the known functions of the PrPc is anti-apoptotic roles preventing neuronal cell death. It has been reported that autophagy is activated during amino-acid or serum deprivation and various pathological situations. Autophagy is an intracellular bulk degradation system, which delivers cytoplasmic components to the lysosome/vacuole. To investigate whether PrPc is involved in an autophagic cell death pathway, we compared expression patterns of microtubule-associated protein 1 light chain-3 (LC3), an autophagy marker, in Prnp-deficient (Prnp-/-) neuronal cells to those with wild-type (WT) neuronal cells. The expression level of LC3-II, an autophagosomal membrane associated form, was increased in the Prnp-/-neuronal cells compare to WT neuronal cells under serum deprivation conditions, but not under normal growth medium conditions. In addition, under serum deprivation conditions, LC3-positive punctated structures were co-localized with lysosomal membrane glycoprotein-2 (LAMP-2), indicating the presence of autophagolysosomes in Prnp-/-neuronal cells. These co-localizations were not observed from WT neuronal cells. Our data suggest that PrPc may play a pivotal role in preventing the autophagic cell death pathway in neuronal cells.
AD J.-M. Oh, E.-K. Choi, Y.-S. Kim, Hallym University, Ilsong Institute of Life Science, Korea; R.I. Carp, New York State Institute for Basic Research in Development Disabilities, USA
SP englisch
PO Schottland