NR AXUD
AU Pastore,M.; Lehmann,S.
TI Surface Scrapie Assay (SSA): A Novel Test to Evaluate the Remaining Prion Infectivity on Contaminated Surfaces Models through Cell Culture
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.123
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
The steel wire test has been applied successfully for the determination of prion residual infectivity, and is recommended to evaluate the efficacy of different cleaning procedures. However its use is subtle and results are often affected by consistent variability; moreover [Lipscomb et al., 2006, J Hosp Infect. 64(4):339-43] demonstrated that the contamination is more easily removed from wires than from flat metal surfaces. Therefore we decided to set up a novel model of cell culture infection to reproduce more realistic conditions representative of surgical instruments in term of dimension, shape and size.
The rationale of the "scrapie surface assay" (SSA) is to contaminate plastic or metal surfaces, in order to consider them as the source of infectivity. Subsequently these surfaces receive prion inactivating treatments. Thereafter cells are seeded at the surface and cell growth and protease K resistance are monitored as for standard infections. Hence this is a more realistic replica to assess the removal or inactivation of the prion infectious agent from medical equipments. Different conditions have been tested to optimize contaminant condition. Plastic and metal material, incubation time, temperature and rinsing condition have been evaluated and screened to select the most performing conditions for the establishment of cellular prion infection. We recently showed [Solassol et al., J Infect Dis. 194:865-9] that a formulation of copper metal ions in combination with hydrogen peroxide has a dramatic reduction effect in vitro on PrPsc present in prion infected brain homogenates including samples from human Creutzfeldt-Jakob disease and in vivo. Procedures derived from the copper and peroxide original formulation have been tested in vitro side by side with the ex vivo systems to compare their feasibility, reproducibility and efficiency.
AD M. Pastore, S. Lehmann, CNRS UPR1142, Institute of Human Genetics, France
SP englisch
PO Schottland