NR AXVM
AU Rybakov,S.; Alekhina,A.; Ryabokon,A.; Metlin,A.; Yegorov,A.
TI BSE Epizootic Situation in the Russian Federation
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.04
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
To assess epidemic situation on bovine spongiform encephalopathy (BSE) specific diagnostic laboratory was established in the FGI "All-Russian Research Institute for Animal Health" in 1997 where diagnostic testings for the disease using histopathological method were started.
During the first years the most part of samples were submitted from various parts of Russia within the programme on passive monitoring. About 500 bovine brain samples were tested annually.
BSE neuropathological signs are very similar to the signs of many diseases including rabies. Since rabies has been registered in some Russian animal farming areas "solid-phase" method for the rabies virus detection in formalin-fixed brain tissues was developed for the differential diagnosis of rabies and BSE in the laboratory.
Synthetic polypeptides - fragments of bovine prion protein and antibodies against them that have sufficient activity and specificity for the PrPBSE detection with immunohistochemical method are prepared. This method is used for the diagnosis confirmation when the result of brain sample test is inconclusive.
According to the OIE recommendations, the programme of active BSE monitoring was started in 2003. It covered mainly the cattle of risk groups: showing the signs of neuropathology, emergency slaughtered, died or imported from BSE-affected European countries.
Since 2004 ELISA on the basis of TeSeE kits (BIO-RAD Co.) has been used in the laboratory for the diagnostic tests of brain samples from cattle imported from BSEaffected countries.
From the beginning of the study till December 31, 2006 3,620 bovine brain samples from 72 Subjects of the Russian Federation were tested. In 2006 550 brain samples were tested, 429 of them were obtained from the cattle of risk groups. In the first quarter of 2007 417 brain samples were tested; all of them were obtained from the cattle of risk groups. No signs typical for BSE (vacuoles or PrPBSE) were detected in the samples tested.
AD S. Rybakov, A. Alekhina, A. Ryabokon, A. Metlin, A. Yegorov, FGI Federal Centre for Animal Health, Russia
SP englisch
PO Schottland