NR AXVR
AU Saifitdinova,A.; Lada,A.; Rubel,A.; Urazalinov,M.; Inge-Vechtomov,S.G.; Galkin,A.
TI Discovery of New Prion-Like Factor in Saccharomyces Cerevisiae
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Natural and Experimental Strains P02.44
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
A new cytoplasmic genetic element has been found in budding yeast S. cerevisiae. This prion-like factor was revealed as a nonsense suppressor in a haploid yeast strain on a background of an unknown chromosomal mutation and substitution of yeast translation termination factor eRF3 with fusion protein Aß40-SUP35MC (Tsaponina et. al, 2005). The system was originally designed for analysis of human beta-amyloid peptide aggregation and the prion-like factor was proposed to affect the aggregation of Aß40-SUP35MCp. We designated this new factor as [ABA+] which stands for Amyloid Beta Aggregase.
[ABA+] is inherited stably through cell generations and demonstrates dominant non-Mendelian inheritance in meiosis. It is curable by yeast antiprion agent GuHCl and as for all known yeast prions [ABA+] propagation depends on the chaperone Hsp104. [ABA+] like another yeast prion [PIN+] is curable by depletion and inactivation of HSP104 but not its overproduction. However, we have shown that [ABA+] is neither [PSI+] nor [PIN+]. Screening of the determinant of [ABA+] is currently underway.
This work is supported by RFBR (07-04-00873a), Russian Ministry of Education and Science (02.512.11.2021, PHII.2.2.2.3.10047) and CRDF (BRHE Y4-B-12-04).
Tsaponina O.E., Lada A.G., Rubel A.A., Navolotskaya V.V., Petrova I.T., Inge-Vechtomov S.G., Galkin A.P. Analysis of effects of Ab-Sup35MC hybrid protein production in yeast Saccharomyces cerevisiae. Ecologicheskaya genetika, 2005, 3(1): 22-33.
AD A. Saifitdinova, A. Rubel, S. Inge-Vechtomov, A. Galkin, Vavilov Institute of General Genetics, Russia; A. Lada, M. Urazalinov, Saint-Petersburg State University, Russia
SP englisch
PO Schottland