NR AXWA
AU Schönenbrücher,H.; Göbel,K.A.; Abdulmawjood,A.; Richt,J.A.; Buelte,M.
TI Detection of Central Nervous System Tissues in Meat Products: Comparison of two ELISAs and a Real Time-PCR
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.21
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
The removal of Central Nervous System (CNS) tissues from the food chain as part of the Bovine Spongiform Encephalopathy (BSE) specified risk material is one of the highest priority tasks to avoid the transmission of BSE to humans. This study compared the diagnostic applicability of a recently developed real time-PCR assay with the Ridascreen(R) Risk material 10/5 ELISA (raw meat), the Ridascreen(R) Risk material ELISA (heat treated meat products) and the ScheBo Brainostic GFAP ELISA-
Kit. Artificially contaminated minced meat as well as different types of moderate and strong heat treated cooked sausages ("Leberwurst-Kesselkonserve", "Dreiviertel- and Vollkonserven") were prepared as internal reference material. The detection limit for bovine brain material was examined throughout a storage time of 14 days for fresh minced meat, 12 months for frozen minced meat and 24 months for the heat treated meat products. The real time-PCR method and the ELISA kits proved to be suitable for the detection of 0.1% CNS tissues in fresh and frozen minced meat. Correct results were obtained even at the end of the storage periods for the different storage conditions. Bovine brain containing heat treated meat products could be reliable identified with all three test systems used. Based on the comparative study and a conducted multi center trial, the real time-PCR assay has been proven to be useful as a routine diagnostic test for the detection of CNS tissues in meat and meat products.
AD H. Schoenenbruecher, J.A. Richt, National Animal Disease Center, USA; K.-A. Goebel, A. Abdulmawjood, M. Buelte, Justus Liebig University of Giessen, Germany
SP englisch
PO Schottland
EA pdf-Datei und Poster (Der rechte Rand des Posters und ein Teil seines Textes sind abgeschnitten)