NR AXWT
AU Sohn,H.J.; Lee,S.Y.; Choi,Y.P.; Cho,I.S.; Joo,Y.S.; Lee,Y.H.; Kim,C.L.; Tark,D.S.
TI Amino Acid Polymorphisms of PrP Gene in Korean Native Goats (Capra Aegagrus Hircus)
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.41
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Scrapie is a fetal neurodegenerative disease of sheep and/or goats which belongs to transmissble spongiform encephalopathies. Scrapie has never been reported in Republic of Korea. Polymorphism in the amino acid sequence of prion protein(PrP) plays a significant role in susceptibility or resistance to scrapie following exposure. In Republic of Korea, 522,534 goats (mostly Korean native goats) and 1,202 sheep were reared according to the Ministry of Agriculture and Forestry data in 2005. Unlike most European countries, goats are more important small ruminants in Republic of Korea. In goats, PrP amino acid dimorphism at codon 142, 143, and 240 has been described. Only dimorphism at codon 142 was associated with an incubation period. However, another PrP variants containing only three instead of the usual five octapeptide repeats, with additional Tryptophan to Glycine substitution in codon 102 may also be associated with an increased incubation period of scrapie in goats. In this study we investigated the PrP polymorphisms in Korean native goats. This study has examined the PrP genotypes of 60 goats raised in Namwon branch station of National Livestock Research institute(NLRI). The genomic DNA was isolated from goat whole blood and the PrP gene including the whole open reading frame(ORF) 794bp was amplified using PCR. Generated PCR fragments of goat PrP gene was sequenced using an automated DNA sequencing device(model 377 DNA sequencer). We found the well-known polymorphism at codon 102, 143, 240 and the existence of known silent alteration at codon 42, 138 in korean native black goats. 15% of tested all goats had Arginine/Arginine at codon 143. It need further research to know whether these novel polymorphisms affects susceptibility to scrapie.
AD H.-J. Sohn, Y.-S. Joo, Y.-H. Lee, C.-L. Kim, D.S. Tark, National Veterinary Research and Quarantine Serive, Foreign Animal Disease Divison, South Korea; S.-Y. Lee, Y.-P. Choi, I.-S. Cho, National Veterinary Research and Quarantine Serive, South Korea
SP englisch
PO Schottland
EA pdf-Datei und Poster (Postertitel: Prion protein gene polymorphism in Korean native goats)