NR AXXB
AU Stadnyk,V.V.; Mayor,C.Y.; Vlizlo,V.V.
TI Expression of PRPC Isoforms in the Parts of Cattle Brain
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.111
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
Background: Prion diseases appear as a result of normal physiological protein PrPc conversation into the abnormal, disease-causing form PrPsc. The physiological form of prion protein is produced naturally in different cells of all mammals, and is harmless. The normal and abnormal prion proteins differ only in their three-dimensional structures. Once the normal protein has undergone such conversation, it can then transform other normal protein molecules into the pathological form. The PrPc expression level is a decisive factor in prion diseases progression, so far as physiological prion protein is a matrix for PrPsc appearance
Aim: The aim of present work was to investigate the level of PrPc isoforms expression in several parts of cattle brain.
Methods: We used Dot blot and disc-electrophoresis in PAAG gradient with Westernblot analysis to study general level of PrPc expression in the parts of cattle brain: in frontal pole of cerebrum, olfactory tract, olfactory cortex, mammillary body, cerebellum, epiphysis cerebri and medulla oblongata.
Results: The glycoform signature of PrPc is in general characterized by the presence of three bands with decreasing intensity, representing the bi-, mono- and unglycosylated isoforms of this protein. We detected two additional isoforms in olfactory tract, olfactory cortex, mammillary body and cerebellum. In frontal pole matter of cerebrum and epiphysis cerebri we detected three additional isoforms. Taking into consideration their electrophotetic mobility these isoforms represent partly glycosylated forms of prion protein.
Conclusion: It was demonstrated that the highest level of this protein posses in medulla oblongata, olfactory tract and olfactory cortex. It was shown that correlation between PrPc glycoforms is rather divers in different parts of cattle brain.
AD Vitalii V. Stadnyk, Chrystyna Y. Mayor, Vasyl V. Vlizlo, Institute of Animal Biology, Scientific Center for Prion Infections Study, Ukraine
SP englisch
PO Schottland