NR AXXK
AU Tamura,Y.; Muramatsu,Y.; Ogawa,T.; Suzuki,K.; Kanameda,M.; Horiuchi,M.
TI Frequencies of PRNP Gene Polymorphisms in Dairy Cattle in Vietnam and Thailand for Potentail Association with BSE
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.76
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB In 2004, Sander et al. found significant associations between bovine spongiform encephalopathy (BSE) susceptibility in German cattle and frequencies of insertion/deletion (indel) polymorphisms within the bovine PRNP gene (Neurogenetics. 5:19-25, 2004). In this study, we investigated the frequencies of indel polymorphisms within two variable sites in the bovine PRNP gene in 206 dairy cattle in Vietnam and in 102 dairy cattle in Thailand. Two variable sites, a 23-bp indel polymorphism (123 bp/100 bp) in the promoter region, and a 12-bp indel polymorphism (103 bp/91 bp) in intron 1, were investigated by PCR. As for the 23-bp indel in Vietnamese dairy cattle, the frequency distributions of the 100-bp allele and 100-bp/100-bp genotype, which were shown to be associated with BSE susceptibility in BSE-affected German cattle, were significantly high (84.7% and 70.7%, respectively). On the other hand, the frequency of the 103-bp allelic polymorphism was higher than that of the 91-bp allelic polymorphism in the 12-bp indel site in Vietnamese cattle (52.3% and 47.7%, respectively). Besides, As for the 12-bp indel in Thailand dairy cattle, the frequency distributions of the 103-bp allele and 103-bp/103-bp genotype were significantly high (84.8% and 72.5%, respectively). Conversely, the frequency of the 103-bp allelic polymorphism was reported to be lower than that of the 91-bp allelic polymorphism in German cattle (49% and 51%, respectively, in healthy cattle and 33% and 67%, respectively, in BSE-affected cattle). A high frequency distribution of the 91-bp allelic polymorphism was reported to be associated with BSE susceptibility. It has remained unclear how the haplotype consisting of an extremely high frequency of the 100-bp allele on the 23-bp indel polymorphism and a low frequency of the 91-bp allele on the 12-bp indel polymorphism in the PRNP gene affects the susceptibility to BSE in Vietnamese dairy cattle.
AD Y. Tamura, Y. Muramatsu, Rakuno Gakuen University, Veterinary Public Health, Japan; T. Ogawa, K. Suzuki, M. Kanameda, Japan International Cooperation Agency, Japan; M. Horiuchi, Hokkaido University, Laboratory of Prion Diseases, Japan
SP englisch
PO Schottland