NR AXYE
AU Vidal,E.; Tortosa,R.; Costa,C.; Alamillo,E.; Torres,J.M.; Ferrer,I.; Pumarola,M.
TI Stress Response in the CNS of a Transgenic Mouse Model of BSE
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.18
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
An immunohistochemical study was performed to evaluate the stress related proteins heat shock protein 25 (HSP25) and metallothionein I+II (MT-I+II) in the brains of a murine model of bovine spongiform encephalopathy (BSE). Transgenic mice (BoTg110) expressing the bovine cellular prion protein were intracerebrally inoculated with brainstem homogenate from BSE infected cattle. PrPBSE deposition in the brain was found as early as 150 days post-inoculation. Mice sacrificed at a terminal stage of the disease were also evaluated. A thorough neuropathological characterisation of this murine model of BSE was conducted, evaluating the presence of spongiform change, disease associated deposits of prion protein and glial response. Such alterations, common in the different known TSEs, were found to be associated with an increase in the glial immunostaining of both HSP25 and MT-I+II. These proteins are associated with cellular stress, particularly oxidative stress phenomena and heavy metal metabolism, mechanisms which seem to have a relevant role in the pathogenesis of BSE.
This study was financed by the project EET2002-05168-C04 of the Spanish Ministry of Science and Technology (MCyT).
AD E. Vidal, M. Pumarola, CReSA, Priocat Laboratory, Spain; R. Tortosa, C. Costa, Veterinary Faculty, UAB, Spain; E. Alamillo, J.M. Torres, INIA, CISA, Spain; I. Ferrer, Bellvitge Teaching Hospital, Spain
SP englisch
PO Schottland