NR AXZF
AU Yamamoto,T.; Ushiki,Y.; Hara,S.; Hall,W.W.; Tsukagoshi-Nagai,H.; Yokoyama,T.; Tagawa,Y.; Sata,T.; Yamakawa,Y.; Kinoshita,N.; Hattori,S.; Irie,S.
TI An Advantageous Method Utilizing the BioMasher and a Sensitive ELISA to Accurately Detect Bovine Spongiform Encephalopathy and Ovaine Scrapie from Brain Tissue
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.184
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB
We developed a new screening method to detect bovine spongiform encephalopathy (BSE). Our method is advantageous because it has a simpler and safer protocol than
commercial kits. We developed a new device, termed the BioMasher, to homogenize brain tissue by passing it through a porous rigid polypropylene filter. A purification step is not involved in the sample preparation. Thus, the time needed for sample pretreatment is substantially shortened, and the risk of infection during sample processing is effectively reduced. We created monoclonal antibodies to prion protein, and used them to construct a sensitive sandwich enzyme-linked immunosorbent assay system. The sensitivities of this assay kit for BSE-positive brain and scrapie brain are comparable or more sensitive than commercial kits. Key words: BSE, ELISA, screening kit
AD Takuji Yamamoto, Y. Ushiki, S. Hara, S. Hattori, S. Irie, Nippi Research Institute of Biomatrix, Japan; W.W. Hall, University College Dublin, Ireland; H. Tsukagoshi-Nagai, Immuno-Biologuical Laboratories Inc, Japan; T. Yokoyama, Y. Tagawa, National Institute of Animal Health, Japan; T. Sata, Y. Yamakawa, National Institute of Infectious Diseases, Japan; N. Kinoshita, Immuno-Biological Laboratories Co, Japan
SP englisch
PO Schottland