NR AXZG
AU Yan,Z.X.; Stitz,L.; Gaedt,S.; Roth,K.; Mauz,P.S.
TI Evaluation of Prion Inactivation by a Novel Test Procedure
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Epidemiology, Risk Assessment and Transmission P04.120
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB A radionuclide method has been developed to validate contamination and remaining contamination after e.g. cleaning and decontamination of surgical instruments. After mock contamination with radioactively labelled blood, the instruments are screened by use of a gamma camera before and after the cleaning procedure. This method provides a simple and non- destructive way to localise and measure remaining contamination precisely, which is especially useful for the study of the reprocessing of instruments with channels and cavities such as endoscopic instruments. Using an experimental model, pathological prion protein, the agent causing transmissible spongiform encephalopathies (TSE), was found to bind tightly to surgical steel surfaces. This intensive binding of the prion protein to steel results in considerable problems to clean these surfaces, a fact relevant to the threat of iatrogenic transmission of e.g. Creutzfeld-Jacob Disease (CJD). In search for new chemical and physical decontamination procedures, the question of whether prion proteins are washed away from the surface of the instrument, or whether it is denatured and infectivity destroyed has to be answered. In order to establish a valid test procedure, we employed the radionuclide method in which purified pathological prion proteins are radioactively labelled and these are used as the test soil to contaminate model instruments such as steel wires. After submitting steel wires to cleaning and decontamination procedures, the remaining radioactivity will be determined and the wires will be used in a bioassay to evaluate a possible correlation between remaining radioactivity and survival time of the experimental animals, determining prion infectivity. The combination of these test methods will help to differentiate between removal and inactivation of pathological prion proteins on surgical steel surface for any given reprocessing procedure.
AD Z.X. Yan, S. Gaedt, P.S. Mauz, HNO Clinic, Germany; L. Stitz, Friedrich Löffler Institut, Institut für Immunologie, Germany; K. Roth, SMP GmbH Pruefen Validieren Forschen, Germany
SP englisch
PO Schottland