NR AXZJ
AU Yoo,H.S.; Herbst,A.; Sullivan,L.; Vanderloo,J.; Aiken,J.M.
TI Cuprizone as an Experimental Control for Prion-Specific Gene Expression Profiling
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.96
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Identification of genes expressed in response to prion infection may help elucidate biomarkers for disease progression, agent replication and mechanisms of neuropathology. In this study, brain gene expression from C57/Bl6 mice infected with RML agent and age-matched controls were profiled using Affymetrix gene arrays. Expression of transcripts was confirmed by qPCR. Profiles were analyzed by robust multi-array analysis. Of the 39,000 genes whose expression was measured, 139 transcripts were up-regulated at end stage while 71 were down-regulated. Functional gene ontology was used to identify processes that changed in response to prion disease. The majority of the cellular processes identified related to the characteristic neuro-inflammation known to occur during prion disease progression. Cuprizone, a copper chelator, mimics the neuro-inflammation observed in prion disease, causing both spongiform change and astrocytosis. Cuprizone treatment induced spongiosis as well as astrocyte proliferation as indicated by GFAP transcriptional activation and immunohistochemistry. We hypothesize that by utilizing a 0.4% cuprizone diet as a control treatment for comparative expression profiling, prion specific responses may be delineated identifying processes specific to cellular pathology and cellular conversion.
AD H.S. Yoo, Seoul National University, Korea; A. Herbst, L. Sullivan, J. Vanderloo, J. Aiken, University of Wisconsin, Comparative Biosciences, USA
SP englisch
PO Schottland