NR AXZQ
AU Zhang,W.; Becker,K.; Karch,H.; Kuczius,T.
TI Development of a Quantitative Immuno-PCR Assay and its use to Detect Very Low Amounts of Prion Proteins
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Pathology and Pathogenesis P03.77
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB Prion diseases are fatal neurological disorders that occur in humans and animals. BSE is the disease of cattle, scrapie of sheep and Creutzfeldt-Jakob of humans. The main characteristic of the disease is the accumulation of an abnormal prion protein (PrPsc). This infectious protein is the converted isoform from a host encoded prion protein (PrPc). The disease is proofed by post mortem detection of PrPsc. Cattle older than three years are routinely examined on BSE by immunoblot and ELISA analysis. However, sensitivity and specifity are the limiting factors of these techniques referring to sufficiently accumulated PrPsc in brain. A sensitive proof to detect very low amounts of PrPsc in tissues, body fluids, blood and in the environment does still not exist, and there is great demand on developing new techniques. We established a very sensitive and specific quantitative immuno-PCR assay detecting physiological prions. This method combines the enzyme-linked immunosorbent assay (ELISA) and an amplification by PCR. The specific signals are intensified by amplification of bound target DNA molecules using the PCR technique. This assay attained a 1000-fold increased sensitivity compared with results of the ELISA technique. The use of the high-sensitive quantification immuno-PCR method with a significant increase of the efficiency in the prion protein analytic may be achievable as an early stage diagnosis of prion diseases and of product contamination.
AD W. Zhang, H. Karch, T. Kuczius, University Hospital Münster, Institute for Hygiene, Germany; K. Becker, University Hospital Münster, Institute for Medical Microbiology, Germany
SP englisch
PO Schottland