NR AXZR
AU Zheng,M.; Qing,L.; Huang,S.; Chen,F.; Wang,M.; Wang,L.; Miller,M.; Hamir,A.N.; Richt,J.A.; O'Rourke,K.; Belay,E.D.; Schonberger,L.B.; Gambetti,P.; Kong,Q.
TI Assessment of Direct and Indirect Transmission of CWD from Three Cervid Species to Humans
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Oral Abstracts FC5.8
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Vortrag
AB Chronic wasting disease (CWD) is the prion disease widespread in cervids (white-tailed deer, mule deer, elk, and moose). The presence of significant prion infectivity reported in the muscles of CWD-affected cervids indicate that people who had consumed venison from CWD-affected animals in the United States and elsewhere may have been exposed to CWD. In order to determine whether the CWD prion, like the bovine spongiform encephalopathy, is transmissible from cervids to humans, cervidized transgenic mice (Tg12) and humanized transgenic mice (Tg1 and Tg40) were created, which express the elk prion protein (PrP) and human PrP in a mouse PrP-null background respectively. The cervidized Tg12 mice intracerebrally inoculated with CWD isolates from elk, mule deer, and white-tailed deer became infected with relatively short average incubation times (118±6 days for elk 1, 142±7 days for elk 2, 187±18 days for mule deer, and 180±3 days for white-tailed deer). The humanized Tg40 mice and Tg1 mice similarly inoculated with human sCJDMM1 became infected with an average incubation time of 263±13 days for Tg40 mice and 266±5 days for Tg1 mice. In contrast, all of the humanized transgenic mice intracerebrally inoculated with CWD isolates from the three cervid species failed to develop the hallmarks of CWD throughout their natural lifespan. Since the host range of a prion strain could change after adaptation in another species and CWD has been transmitted to cattle and sheep after experimental inoculation, humanized and cervidized Tg mice were also inoculated with cattle- or sheep-adapted CWD, but the mice failed to become infected so far. Our data point to the existence of a significant species barrier between CWD and humans, which may strongly limit the human transmissibility of CWD. Supported by NINDS NS052319, NIA AG14359, CDC UR8/CCU515004 and Charles S. Britton Fund. The first three authors contributed equally.
AD M. Zheng, L. Qing, S. Huang, F. Chen, M. Wang, L. Wang, P. Gambetti, Q. Kong, Case Western Reserve University, Pathology, USA; M. Miller, Colorado Division of Wild Life, Wildlife Research Center, USA; A.N. Hamir, J.A. Richt, USDA, National Animal Disease Center, USA; K. O'Rourke, USDA ARS, Animal Disease Research Unit, USA; E.D. Belay, L.B. Schonberger, Centers for Disease Control and Prevention, National Center for Infectious Diseases, USA
SP englisch
PO Schottland