NR AXZV
AU Zurzolo,C.; Marijanovic,Z.; Campana,V.
TI Subcellular Compartments of Scrapie Production
QU International Conference - Prion 2007 (26.-28.9.2007) Edinburgh International Conference Centre, Edinburgh, Scotland, UK - Book of Abstracts: Protein Misfolding P01.05
IA http://www.prion2007.com/pdf/Prion Book of Abstracts.pdf
PT Konferenz-Poster
AB It has been suggested that the trafficking of PrPc plays an essential role in its conversion to PrPsc. Thusfar it has been reported that surface expression of PrPc is important for scrapie production; it has also been demonstrated that this conversion event requires that the protein enter the endocytic pathway. However, the precise subcellular compartment in which the prion conversion event occurs has not been identified. Several lines of evidence suggest that the late endosomal/lysosomal compartment is a likely location for scrapie production since in some cell lines PrPsc has been found to accumulate in late endosomes. Conversely, other studies implicate the Golgi as a candidate for scrapie accumulation. Despite these observations, none of these organelles has been demonstrated to be directly involved in the conversion of PrPc to PrPsc. Using different approaches we perturbed trafficking between different endocytic compartments and analyzed their impact on PrPsc production. Interestingly, a significant amount of PrPsc is localized in endosomal recycling compartment. Our results suggest that neither early nor late endosomes represent major sites of PrPsc production but implicate the endosomal recycling compartment in scrapie production.
AD C. Zurzolo, Z. Marijanovic, V. Campana, Pasteur Institute, Cell Biology and Infection, France
SP englisch
PO Schottland
EA pdf-Datei und Poster, das eine geänderte Autorenliste aufweist