NR AXZX
AU Adler,V.; Davidowitz,E.; Tamburi,P.; Rojas,P.; Grossman,A.
TI Alpha2-macroglobulin is a potential facilitator of prion protein transformation
QU Amyloid 2007 Mar; 14(1): 1-10
PT journal article; research support, n.i.h., extramural
AB Cellular prion protein changes conformation during transformation to an infectious scrapie isoform. One measure of transformation is the development of partial resistance to protease treatment. A fraction of human and bovine plasma was identified containing activity that facilitates transformation of cellular prion protein to a protease resistant isoform in the presence of RNA in the absence of seeded scrapie prion protein. Purification of proteins from this fraction led to the identification of alpha2-macroglobulin as an active component suggesting that it may facilitate conformational changes in prion protein in spontaneous forms of prion disease.
MH Animals; Cricetinae; Humans; Microscopy, Electron; Prions/*metabolism/ultrastructure; Recombinant Proteins/isolation & purification/metabolism; alpha-Macroglobulins/isolation & purification/*metabolism
AD Q-RNA, Inc., Audubon Business and Technology Center. 3960 Broadway, Suite 440C, New York, NY, 10032. USA.
SP englisch
PO USA