NR AYBS
AU Klajnert,B.; Cangiotti,M.; Calici,S.; Majoral,J.P.; Caminade,A.M.; Cladera,J.; Bryszewska,M.; Ottaviani,M.F.
TI EPR study of the interactions between dendrimers and peptides involved in Alzheimer's and prion diseases
QU Macromolecular Bioscience 2007 Aug 7; 7(8): 1065-74
PT journal article; research support, non-u.s. gov't
AB Spin-probe and spin-label techniques were used to study the interactions of the Abeta 1-28 peptide involved in Alzheimer disease and the PrP 106-126 peptide suspected to be preferentially involved in spongiform encephalopathies with three different types of dendrimers. A computer-aided EPR analysis of a positively charged and a neutral spin probe was performed by comparing the pure dendrimer and peptide systems with the dendrimer-peptide ones. Also spin-labeled PAMAM dendrimers were used to test the interactions. The results show the interactions between dendrimer and peptide monomer to be stronger for Abeta 1-28 than for PrP 106-126. PAMAM dendrimers perturb the aggregation of the peptides more than PPI dendrimers do.
MH Alzheimer Disease/*metabolism; Amyloid beta-Protein/chemistry/*metabolism; Dendrimers/chemistry/*metabolism; Electron Spin Resonance Spectroscopy; Peptides/chemistry/*metabolism; Prion Diseases/*metabolism; Prions/chemistry/*metabolism
AD Department of General Biophysics, University of Lodz, 12/16 Banacha St., 90-237 Lodz, Poland.
SP englisch
PO Deutschland