NR AYBW
AU Kurt,T.D.; Perrott,M.R.; Wilusz,C.J.; Wilusz,J.; Supattapone,S.; Telling,G.C.; Zabel,M.D.; Hoover,E.A.
TI Efficient in vitro amplification of chronic wasting disease PrPres
QU Journal of Virology 2007 Sep; 81(17): 9605-8
PT journal article; research support, n.i.h., extramural
AB Chronic wasting disease (CWD) of cervids is associated with conversion of the normal cervid prion protein, PrPc, to a protease-resistant conformer, PrP(CWD). Here we report the use of both nondenaturing amplification and protein-misfolding cyclic amplification (PMCA) to amplify PrP(CWD) in vitro. Normal brains from deer, transgenic mice expressing cervid PrPc [Tg(cerPrP)1536 mice], and ferrets supported amplification. PMCA using normal Tg(cerPrP)1536 brains as the PrPc substrate produced >6.5 x 10(9)-fold amplification after six rounds. Highly efficient in vitro amplification of PrP(CWD) is a significant step toward detection of PrP(CWD) in the body fluids or excreta of CWD-susceptible species.
MH Animals; Brain/*pathology; Deer; Ferrets; Mice; Mice, Transgenic; Prions/isolation & purification/*metabolism; Wasting Disease, Chronic/*diagnosis
AD Department of Microbiology, Immunology, and Pathology, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
SP englisch
PO USA