NR AYDU
AU Sethi,S.K.; Kerksiek,K.M.; Brocker,T.; Kretzschmar,H.A.
TI Role of the CD8+ dendritic cell subset in transmission of prions
QU Journal of Virology 2007 May; 81(9): 4877-80
PT comparative study; journal article; research support, non-u.s. gov't
AB Controversial results have been observed in mouse models regarding the role of lymphoid tissues in prion pathogenesis. To investigate the role of dendritic cells (DC), we used a transgenic mouse model. In this model (CD11c-N17Rac1), a significant reduction of CD8+ CD11c(hi) DC has been described, and the remaining CD8+ DC demonstrate a reduced capacity for the uptake of apoptotic cells. After intraperitoneal prion infection, significantly longer incubation times were observed in CD11c-N17Rac1 mice than in controls, indicating that a defect in CD8+ CD11c(hi) DC significantly impedes neuroinvasion after intraperitoneal infection. In contrast, no distinct differences were observed between CD11c-N17Rac1 mice and controls after oral infection. This provides evidence that oral and intraperitoneal prion infections differ in lymphoreticular requirements.
MH Administration, Oral; Animals; Antigens, CD11c/metabolism; Antigens, CD8/metabolism; Brain/*metabolism; Dendritic Cells, Follicular/*metabolism; Immunohistochemistry; Injections, Intraperitoneal; Mice; Mice, Transgenic; Prion Diseases/metabolism/*transmission; Prions/administration & dosage/*metabolism
AD Center for Neuropathology and Prion Research, Ludwig Maximilians University, Feodor-Lynen-Strasse 23, 81377 Munich, Germany.
SP englisch
PO USA