NR AYEU
AU Yun,S.W.; Kouznetsova,E.; Nitschke,C.; Heinitz,K.; Schliebs,R.; Gerlach,M.; Riederer,P.; Klein,M.A.
TI ß-Amyloid deposition and prion infection in adult primary brain cell long-term culture model
QU Biochemical and Biophysical Research Communications 2007 Aug 31; 360(3): 520-4
PT journal article; research support, non-u.s. gov't
AB We report an in vitro model of the adult central nervous system produced by culturing primary brain cells isolated from adult mice for periods longer than 4 months. We applied this novel cell culture method to model progressive neurodegenerative diseases. After long-term culture of adult primary brain cells prepared from Alzheimer's disease and prion disease mouse models, we observed beta-amyloid deposition and prion infection in primary cell cultures in vitro.
MH Amyloid beta-Protein/*metabolism; Animals; Brain/*metabolism/*pathology; Cells, Cultured; Disease Progression; Mice; Mice, Inbred C57BL; Models, Biological; Prion Diseases/*metabolism/*pathology; Survival Rate; Time Factors
AD Clinical Neurochemistry and NPF Center of Excellence Laboratories, Clinic of Psychiatry and Psychotherapy, University of Würzburg, 97080 Würzburg, Germany. Seong-Wook.Yun@actelion.com
SP englisch
PO USA