NR AYHN
AU Liao,M.J.; Zhang,C.C.; Zhou,B.; Zimonjic,D.B.; Mani,S.A.; Kaba,M.; Gifford,A.; Reinhardt,F.; Popescu,N.C.; Guo,W.; Eaton,E.N.; Lodish,H.F.; Weinberg,R.A.
TI Enrichment of a population of mammary gland cells that form mammospheres and have in vivo repopulating activity
QU Cancer Research 2007 Sep 1; 67(17): 8131-8
PT journal article; research support, n.i.h., extramural; research support, n.i.h., intramural; research support, non-u.s. gov't; research support, u.s. gov't, non-p.h.s.
AB The identification of mammary gland stem cells (MGSC) or progenitors is important for the study of normal breast development and tumorigenesis. Based on their immunophenotype, we have isolated a population of mouse mammary gland cells that are capable of forming "mammospheres" in vitro. Importantly, mammospheres are enriched for cells that regenerate an entire mammary gland on implantation into a mammary fat pad. We also undertook cytogenetic analyses of mammosphere-forming cells after prolonged culture, which provided preliminary insight into the genomic stability of these cells. Our identification of new cell surface markers for enriching mammosphere-initiating cells, including endoglin and prion protein, will facilitate the elucidation of the cell biology of MGSC.
MH Adult Stem Cells/*cytology/*transplantation; Animals; Cell Culture Techniques; Cell Differentiation; *Cell Proliferation; Cell Shape; Female; Mammary Glands, Animal/*cytology/*transplantation; Mice; Mice, Inbred C57BL; Mice, Knockout; Organoids/cytology
AD Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.
SP englisch
PO USA