AU Lücker,E.H.; Eigenbrodt,E.; Wenisch,S.; Leiser,R.; Bülte,M.
TI Identification of central nervous system tissue in retail meat products
QU Journal of Food Protection 2000 Feb; 63(2): 258-63
PT journal article
AB A procedure to detect tissues from the central nervous system that involved quantification of cholesterol and immunochemical detection of neuron-specific enolase and glial fibrillary acidic protein was used to analyze 402 samples of heat-treated meat products from various food outlets in Germany. The cholesterol content of 16 samples (4.0%) indicated the possible presence of central nervous system tissue because the levels exceeded the normal maximum cholesterol content of cooked sausages. In 7 of these 16 heat-treated meat products, immunoblotting of both neuron-specific enolase and glial fibrillary acidic protein confirmed the presence of CNS tissue. Repeated sampling by veterinary officials and analysis by both cholesterol quantification and immunoblotting confirmed these findings. Whereas all of the control samples (with and without added central nervous system tissue) were correctly classified by both cholesterol quantification and immunoblotting, negative results of immunoblotting must be carefully interpreted in the case of intensively heat-treated meat products. Thus, studies have yet to establish an increase in sensitivity of immunoblotting of neuron-specific enolase and glial fibrillary acidic protein. However, the detection of illegal use of central nervous system tissue in heat-treated retail meat products demonstrates the need for suitable analytical methods to control transmissible encephalopathies and to enforce labeling laws.
AD
Dr. Ernst Lücker, ernst.h.luecker@vetmed.uni-giessen.de ; Fax 0641/99-38259 - Institut für Tierärztliche Nahrungsmittelkunde, Justus-Liebig Universität, Giessen Frankfurter Str. 92, 35392 Giessen, Germany
Prof. Dr. Michael Bülte, Tel. 0641/99-38250 - Institut für Tierärztliche Nahrungsmittelkunde, Justus-Liebig Universität, Giessen Frankfurter Str. 92, 35392 Giessen, Germany
Prof. Dr. Rudolf Leiser, Tel. 0641/99-38100 - Institut für Veterinär-Anatomie, -Histologie und -Embryologie, Justus-Liebig Universität, Giessen Frankfurter Str. 98, 35392 Giessen, Germany
Prof. Dr. Erich Eigenbrodt, Tel. 0641/99-38172 - Institut für Biochemie und Endokrinologie, Justus-Liebig Universität, Giessen Frankfurter Str. 100, 35392 Giessen, Germany
SP englisch
PO USA
ZF kritische Zusammenfassung von Roland Heynkes